Compare polyA and ribo depletion mRNA head to head in nearly 300 matched samples
The RNA atlas that is hosted in the R2 Platform ( https://r2.amc.nl ) is a great 300 samples reseource where all of the samples have been analysed on polyA isolation, ribo depletion as well as small RNA sequencing. As such it is an invaluable resource that can also be used to investigate the effects of the different isolation methods. You can use the 'two-set view' to analyze andvisualize 2 resources head-to-head h For example check out the expression of a Histone gene (that lacks a poly A tail), where you can clearly see that the poly A isolation lacks the aility to assess the expression of such genes. Or visualize the profiles in the landscape views in the embedded genome browser of R2. Just a couple of simple things that R2 can be of great value with a few mouse clicks. Visit https://r2.amc.nl and explore this, or one of the hundreds of other public resources from the comfort of your web browser
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